| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058528 |
| Chromosome: | chromosome 12 |
| Location: | 7268705 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557900 | HYD1,CDI1 | C-8%252C7 sterol isomerase; (1 of 1) 5.3.3.5 - Cholestenol Delta-isomerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATGGACAACGAGTTGGCCAAGGAAAAC |
| Internal bar code: | CGTTGAGTGACTACTTACCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 874 |
| LEAP-Seq percent confirming: | 99.0679 |
| LEAP-Seq n confirming: | 2232 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAATACCACGTTGCGGAGC |
| Suggested primer 2: | TTACGGACTCTTTCCAACGG |