| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058563 |
| Chromosome: | chromosome 12 |
| Location: | 4351038 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520173 | (1 of 1) PTHR10887//PTHR10887:SF340 - DNA2/NAM7 HELICASE FAMILY // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGTTTAATGTCTTTGCCGCGGCTCGTC |
| Internal bar code: | GGTTCCTATGCCACCGTGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 |
| LEAP-Seq percent confirming: | 99.5935 |
| LEAP-Seq n confirming: | 735 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACCGCCATAGATACG |
| Suggested primer 2: | CCTACCTCGAGCCTGTTCAC |