| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058592 |
| Chromosome: | chromosome_5 |
| Location: | 2953558 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g238280 | FAP205 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGGGCTGTGGGTGGATGAGCCCGTACGCA |
| Internal bar code: | GACGCGACGCACCGTCATAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 359 |
| LEAP-Seq percent confirming: | 98.7654 |
| LEAP-Seq n confirming: | 2080 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTATTGAGCGTCCTGCTCC |
| Suggested primer 2: | ACGCACCACCTGAAGAAGAC |