| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058594 |
| Chromosome: | chromosome 12 |
| Location: | 7884443 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552750 | (1 of 2) PF04520 - Senescence regulator (Senescence_reg) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACCGCAACCTTAGTGACGTTGTGAAGC |
| Internal bar code: | GTTTTCCGAAGTTTCTATTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 99.6539 |
| LEAP-Seq n confirming: | 7775 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGACTTGGCCGATGAAG |
| Suggested primer 2: | ACACACACACACACACACCG |