Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.058609 |
Chromosome: | chromosome_11 |
Location: | 331236 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467567 | UBQ1 | Ubiquitin | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAAGCACCGCACACCAGCAACCGGCCCTCG |
Internal bar code: | ACCTTTGATCAATAGGAGTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 678 |
LEAP-Seq percent confirming: | 99.7374 |
LEAP-Seq n confirming: | 4938 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCATTAAGGCGGTGAGAAAA |
Suggested primer 2: | CTCGGATTCCGTGATCTTGT |