Insertion junction: LMJ.RY0402.058616_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.058616
Chromosome:chromosome 14
Location:2804929
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g627350 XYI3 Sulfite exporter family protein; (1 of 4) PTHR14255:SF3 - SULFITE EXPORTER TAUE/SAFE FAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTCACGTGCCCCGGGACCGTCGTCCGCT
Internal bar code:ACTTCACGTCATCCCGCCCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:107
LEAP-Seq percent confirming:99.0164
LEAP-Seq n confirming:1208
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGCGTCTTCACTTGTTCA
Suggested primer 2:ATCTCTCGCAGAGCTCAAGG