| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058649 |
| Chromosome: | chromosome 11 |
| Location: | 2464280 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475550 | UMM10 | (1 of 2) 2.1.1.165 - Methyl halide transferase / Methyl chloride transferase; methyl-halide synthetase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTTAGGCGAAAACCGCTCCTCGACCTC |
| Internal bar code: | CCTTAGCCGCCGAATCGGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 98.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCACCAGTAAGTAACGGA |
| Suggested primer 2: | ACGAAATTGACCGTAGGACG |