| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058655 |
| Chromosome: | chromosome 7 |
| Location: | 2535643 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGACTCGGCAGTGACCGGCGTGCTGAC |
| Internal bar code: | CCCACTTTATGCGGTCTGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 164 |
| LEAP-Seq percent confirming: | 80.3698 |
| LEAP-Seq n confirming: | 1130 |
| LEAP-Seq n nonconfirming: | 276 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGTCGACTTCTGCTGGT |
| Suggested primer 2: | AAAGAGAGGCGGAGAAGGAC |