| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058676 |
| Chromosome: | chromosome 7 |
| Location: | 3741887 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338000 | MCM2 | (1 of 1) K02540 - DNA replication licensing factor MCM2 (MCM2); Minichromosome maintenance protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCGCACCCGCCCTTCCTCCCTCCTTCT |
| Internal bar code: | AGTAACGGTGGGGCGAAATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 97.6107 |
| LEAP-Seq n confirming: | 4167 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACATCTGTTTCCCAACG |
| Suggested primer 2: | CCCTCTCTACTGTTGCTGGC |