| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058703 |
| Chromosome: | chromosome 8 |
| Location: | 3386215 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374300 | CGL115 | (1 of 1) K19323 - ataxin-10 (ATXN10); Conserved in the Green Lineage | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAATCGCCTTGCTCTGAGCTAATTATGG |
| Internal bar code: | GACAGCGTCCGCTCCGGTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 99.365 |
| LEAP-Seq n confirming: | 3599 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAAAACTGTCACCATCC |
| Suggested primer 2: | ACTCGTCACAACCCTAACGG |