Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.058723 |
Chromosome: | chromosome 16 |
Location: | 3855867 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g689950 | (1 of 1) PF00168//PF08372 - C2 domain (C2) // Plant phosphoribosyltransferase C-terminal (PRT_C) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAGCCCCGTGTCGCCATCCAGCTCCAA |
Internal bar code: | AATAACAAAACTTCGACGTACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 575 |
LEAP-Seq percent confirming: | 99.5918 |
LEAP-Seq n confirming: | 732 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACACTGTCCTTGCGCTTTT |
Suggested primer 2: | CAATGCACAACATCACCTCC |