Insertion junction: LMJ.RY0402.058723_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.058723
Chromosome:chromosome 16
Location:3855867
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g689950 (1 of 1) PF00168//PF08372 - C2 domain (C2) // Plant phosphoribosyltransferase C-terminal (PRT_C) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGAGCCCCGTGTCGCCATCCAGCTCCAA
Internal bar code:AATAACAAAACTTCGACGTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:575
LEAP-Seq percent confirming:99.5918
LEAP-Seq n confirming:732
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AACACTGTCCTTGCGCTTTT
Suggested primer 2:CAATGCACAACATCACCTCC