| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058725 |
| Chromosome: | chromosome 6 |
| Location: | 2161494 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265900 | (1 of 1) K07023 - putative hydrolases of HD superfamily (K07023) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCGATCCTTCTCTCACCACGCCCCCCC |
| Internal bar code: | CAGCATTGGGGACCAAAGAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 744 |
| LEAP-Seq percent confirming: | 95.7368 |
| LEAP-Seq n confirming: | 1033 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCTAGGGAGGGGGACAG |
| Suggested primer 2: | TAGCAACAGACTGCCAATGC |