| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058742 |
| Chromosome: | chromosome 10 |
| Location: | 2057381 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433000 | TSG2 | (1 of 1) K14164 - glycyl-tRNA synthetase (glyQS); Glycyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCTTGGCAGGTGCTTGGCAGTAGCCG |
| Internal bar code: | ATTGGACCGTATGGGCCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 99.4592 |
| LEAP-Seq n confirming: | 1839 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCCCTAGCCACTACAATC |
| Suggested primer 2: | CAACTTCAAGGACATCCGGT |