| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058749 |
| Chromosome: | chromosome 1 |
| Location: | 6876862 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049750 | (1 of 1) PTHR12714:SF11 - C-TERMINAL S-ISOPRENYLCYSTEINE CARBOXYL O-METHYLTRANSFERASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGGGCCTGCAGGCGCCTGAGAGCATCC |
| Internal bar code: | ATGCCCTGTAGGGAGCGTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 99.2138 |
| LEAP-Seq n confirming: | 3786 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTAAATCCTGCGTCACTG |
| Suggested primer 2: | GTTGCACCTGTTTGGAACCT |