Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.058754 |
Chromosome: | chromosome 2 |
Location: | 4082329 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g097500 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGATATCGCGGGGCAGGCGTATCCGCC |
Internal bar code: | CTGCCGTAAGGACATCGGAGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 866 |
LEAP-Seq percent confirming: | 97.8492 |
LEAP-Seq n confirming: | 18744 |
LEAP-Seq n nonconfirming: | 412 |
LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAACCTGCTACGGTGTTTT |
Suggested primer 2: | AATCGAGTCCAGTTCCATGC |