Insertion junction: LMJ.RY0402.058755_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.058755
Chromosome:chromosome 13
Location:1166208
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g569651 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGATGTGAGTGGTAATAGGTGCCATCATT
Internal bar code:TATTGTCAGTGTTAGTTTTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:242
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTGCTCTCTCTCTCTGA
Suggested primer 2:TTTGTCTTCCTGCTCCTCGT