| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058764 |
| Chromosome: | chromosome_13 |
| Location: | 2066536 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g577352 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTAGTAGTCTGGCTGCTGATGTCCTCATCT |
| Internal bar code: | CTGTGGGAAAGCCAATCTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 98.9705 |
| LEAP-Seq n confirming: | 3461 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTGACGGACATAATGGA |
| Suggested primer 2: | TGGCGTCAATAGTGAGCTTG |