| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058773 |
| Chromosome: | chromosome 3 |
| Location: | 3594479 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168400 | OPR134 | OctotricoPeptide Repeat protein 134 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTGTGACCACACCTTCTCTCGGGTGCAG |
| Internal bar code: | ATCTAGCCCCCAGCGGTTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 99.3895 |
| LEAP-Seq n confirming: | 14652 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTGACCATGAAGTGGAA |
| Suggested primer 2: | GTACGCATACGAAGCTGCAA |