| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058774 |
| Chromosome: | chromosome_6 |
| Location: | 3930401 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g278186 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGACGCCACGGTCTGGCACGGCTGCCGCTG |
| Internal bar code: | AATATATGATTCGGGTAGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 99.8682 |
| LEAP-Seq n confirming: | 758 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGATGGGCAGAATGTGT |
| Suggested primer 2: | AAGGTGGTTGTCCTCATTGC |