| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058780 |
| Chromosome: | chromosome 7 |
| Location: | 896187 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318850 | HSP22B | heat shock protein 22B; (1 of 2) K13993 - HSP20 family protein (HSP20) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATAAGAGACATGGAGTCATGGATGCGCGG |
| Internal bar code: | TTAATTTTAGGTACTGTCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1117 |
| LEAP-Seq percent confirming: | 99.4433 |
| LEAP-Seq n confirming: | 34653 |
| LEAP-Seq n nonconfirming: | 194 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTGTGTGAGCCTTCTGA |
| Suggested primer 2: | GGCATCACGATAATGGCTCT |