Insertion junction: LMJ.RY0402.058789_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.058789
Chromosome:chromosome 13
Location:2788248
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g582800 (1 of 1) 6.3.2.11 - Carnosine synthase / Carnosine synthetase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACCTGCCCACCACCCTCGCCCCCGCTT
Internal bar code:TTAGAAAGGGCAATTTCCTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:653
LEAP-Seq percent confirming:73.8358
LEAP-Seq n confirming:4408
LEAP-Seq n nonconfirming:1562
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTTGGGAAGGTAGCTGGT
Suggested primer 2:ATGAAGCGCACAGAGTGATG