Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.058789 |
Chromosome: | chromosome 13 |
Location: | 2788248 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g582800 | (1 of 1) 6.3.2.11 - Carnosine synthase / Carnosine synthetase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACCTGCCCACCACCCTCGCCCCCGCTT |
Internal bar code: | TTAGAAAGGGCAATTTCCTATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 653 |
LEAP-Seq percent confirming: | 73.8358 |
LEAP-Seq n confirming: | 4408 |
LEAP-Seq n nonconfirming: | 1562 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGTTGGGAAGGTAGCTGGT |
Suggested primer 2: | ATGAAGCGCACAGAGTGATG |