| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058808 |
| Chromosome: | chromosome 14 |
| Location: | 3386466 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630301 | (1 of 1) IPR000104//IPR001890//IPR029058 - Antifreeze protein, type I // RNA-binding, CRM domain // Alpha/Beta hydrolase fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAATATCAGAGGCGGGGGAGGCAGCGGC |
| Internal bar code: | TGTTGTGTGTTGCCAGGGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 429 |
| LEAP-Seq percent confirming: | 42.7518 |
| LEAP-Seq n confirming: | 18196 |
| LEAP-Seq n nonconfirming: | 24366 |
| LEAP-Seq n unique pos: | 114 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | GTCTGGTGGGTGCTGTTTTT |