| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058813 |
| Chromosome: | chromosome 12 |
| Location: | 7146583 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559000 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCGGATGTACACACCGCTGAGGTCCAC |
| Internal bar code: | TAAATGACTGGTTTCCGCCTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 99.1416 |
| LEAP-Seq n confirming: | 3234 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAAGCGCGCGTACATAGA |
| Suggested primer 2: | ACGTAAACCCGAAACACTGC |