Insertion junction: LMJ.RY0402.058817_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.058817
Chromosome:chromosome 1
Location:2960718
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g018100 (1 of 1) 3.1.3.16//3.1.3.27//3.1.3.48 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase // Phosphatidylglycerophosphatase // Protein-tyrosine-phosphatase / PTPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATGCCAAGGCAGCGCAGACCTGGACCTA
Internal bar code:GTATAAAGTCGCGTGCGGACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:542
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1191
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCTCGCTCTTGTACTTTG
Suggested primer 2:CAGTATGGTGCATCTGGTGG