| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058819 |
| Chromosome: | chromosome 11 |
| Location: | 3184640 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479383 | (1 of 1) IPR000104//IPR013122 - Antifreeze protein, type I // Polycystin cation channel, PKD1/PKD2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGTACGAAACGCGTCGTGCGCAATACAG |
| Internal bar code: | ACAGTGTTGGTCTAGACTCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 98.9011 |
| LEAP-Seq n confirming: | 180 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAAGGTCACACGCGATCC |
| Suggested primer 2: | GGGTTGCAGGTGTGTATCCT |