| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058850 |
| Chromosome: | chromosome 10 |
| Location: | 2577742 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436950 | Putative nucleolar protein; (1 of 4) PF07534 - TLD (TLD) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCAGCGCGCGGCTGCCGCCGGGTGAGG |
| Internal bar code: | CTGTCAGGCTGGATGCAGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1012 |
| LEAP-Seq percent confirming: | 99.6794 |
| LEAP-Seq n confirming: | 23940 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCTAACTACGAGAACCC |
| Suggested primer 2: | TGTCCACATGCAGATACGGT |