| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058855 |
| Chromosome: | chromosome 10 |
| Location: | 5312245 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCAGTGACCAGTCCGATCATCACCAGCT |
| Internal bar code: | CTGGGACACAGCGTTCCATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 97.3081 |
| LEAP-Seq n confirming: | 2675 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCTCGTTCCTCAGTCAA |
| Suggested primer 2: | AGCTCAGCGAGTGGTGGTAT |