| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058885 |
| Chromosome: | chromosome 1 |
| Location: | 6993670 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050500 | PPR1 | Pentatrichopeptide repeat protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGCGTACACGTTATGGGCTGTAGCACA |
| Internal bar code: | GGGTTTGTTTGCCGGGCCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 79.7297 |
| LEAP-Seq n confirming: | 177 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTCTGTGGCTCTGTCTT |
| Suggested primer 2: | GCTAGAGTATGACCTGCGGC |