| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058913 |
| Chromosome: | chromosome_4 |
| Location: | 2663157 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g223100 | CAH1 | Carbonic anhydrase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCAATCCAATGGAACTCAGCACGCATGAC |
| Internal bar code: | CCGCACCGTAGGAGGCGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 95.0581 |
| LEAP-Seq n confirming: | 981 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTTGTAGCTACCTCGCTC |
| Suggested primer 2: | TACGAAGCTGTCACGGTTTG |