| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058941 |
| Chromosome: | chromosome 6 |
| Location: | 7378887 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299300 | TUG,TUG1 | Gamma tubulin; (1 of 1) K10389 - tubulin gamma (TUBG) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAGGGGTGCAGCAGTAGGGGTACGCAT |
| Internal bar code: | TGAAGAATCACCCTAGTGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 918 |
| LEAP-Seq percent confirming: | 99.161 |
| LEAP-Seq n confirming: | 1891 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATACGGTCCCGCGTCTTA |
| Suggested primer 2: | GGTGGTAAGAGGCGTGTCAT |