Insertion junction: LMJ.RY0402.058962_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.058962
Chromosome:chromosome 11
Location:1293519
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467710 VSP1 Hydroxyproline-rich cell wall glycoprotein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACAGCAAGGACAGCCCCGGCCCAGGGTC
Internal bar code:CAGTAGCGAGTTTAGAAGGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1176
LEAP-Seq percent confirming:98.5366
LEAP-Seq n confirming:202
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGCTGCCTTCTGTCCTAC
Suggested primer 2:TGAGCCTGAGGTTGAGGACT