| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058970 |
| Chromosome: | chromosome 13 |
| Location: | 4208374 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592450 | COPE1 | (1 of 1) K17268 - coatomer protein complex, subunit epsilon (COPE); Epsilon subunit of COP-I complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGCCCGCTTATGCAATGCTGGCTGCC |
| Internal bar code: | TTCATACAGGCATCGCTACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 732 |
| LEAP-Seq percent confirming: | 99.2138 |
| LEAP-Seq n confirming: | 1262 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAAGATTTGGGTGAAGGC |
| Suggested primer 2: | TCTGGGAGACATATCGGGAG |