| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058991 |
| Chromosome: | chromosome 6 |
| Location: | 8979755 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311600 | RFK3 | Riboflavin kinase; (1 of 1) PTHR22749//PTHR22749:SF1 - RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTGGCCTTAACATCATCTTGAACGTCC |
| Internal bar code: | AGACGCATTTGAGGTCGGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 686 |
| LEAP-Seq percent confirming: | 99.3945 |
| LEAP-Seq n confirming: | 1149 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCACAAGACGGTGAGAGG |
| Suggested primer 2: | CACCTGTTTCTTCCCGTGTT |