| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059003 |
| Chromosome: | chromosome 4 |
| Location: | 1760946 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212200 | (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTAAGGGCTGTGCGCGCAGGGGCAGGC |
| Internal bar code: | ACCGTCTGCTGGACTGCCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 99.5223 |
| LEAP-Seq n confirming: | 3125 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGCCTCCTCCTTCCTCT |
| Suggested primer 2: | GGGATCCATCATGCCAATAC |