| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059030 |
| Chromosome: | chromosome_9 |
| Location: | 3325961 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g388393 | ELG13 | Exostosin-like glycosyltransferase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACTACACTTAATAAGCGCGCTGGGGCGGGA |
| Internal bar code: | CTAGAGTGCTTTCATAACCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1313 |
| LEAP-Seq percent confirming: | 47.6695 |
| LEAP-Seq n confirming: | 450 |
| LEAP-Seq n nonconfirming: | 494 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCAAGAAGGGACAAG |
| Suggested primer 2: | TAGTTTTTGTGGTTGCGCTG |