| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059033 |
| Chromosome: | chromosome 12 |
| Location: | 5880974 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCACACACCAACACCCCAGACAGACAT |
| Internal bar code: | TTATGTGGCCGCCCGAGTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1037 |
| LEAP-Seq percent confirming: | 99.8522 |
| LEAP-Seq n confirming: | 3377 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGATCGTCAAGTGCTGCAT |
| Suggested primer 2: | GAGGAGCTGGCACATTAAGC |