| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059044 |
| Chromosome: | chromosome 5 |
| Location: | 2611641 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236250 | Conserved WD40-repeat Protein; (1 of 2) PTHR22847//PTHR22847:SF459 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGAACGCCAGACAAGCCAGGGTGGTCT |
| Internal bar code: | GGGCCCTTATAGGTTGACCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 57.617 |
| LEAP-Seq n confirming: | 1354 |
| LEAP-Seq n nonconfirming: | 996 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAAGGTATGTGCAATCT |
| Suggested primer 2: | ATCCTCCTCCTCCTCGTCAT |