Insertion junction: LMJ.RY0402.059055_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.059055
Chromosome:chromosome 2
Location:5320187
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g106950 (1 of 6) PTHR22916//PTHR22916:SF9 - GLYCOSYLTRANSFERASE // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGATCCGCCTGAACATGATCCATTCCT
Internal bar code:TTCGAGCTAATTTTTATGTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:440
LEAP-Seq percent confirming:99.6453
LEAP-Seq n confirming:13205
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGCGACATGTTTTACAGG
Suggested primer 2:CCAATCAACACAGAACACCG