| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059106 |
| Chromosome: | chromosome 6 |
| Location: | 8214043 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305600 | ADH9 | Alcohol dehydrogenase; (1 of 1) 1.1.1.329 - 2-deoxy-scyllo-inosamine dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACCGGGTGGGTGCAGGCCTGCAGGGGC |
| Internal bar code: | GGGTTGCGCTAAGAACACGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 98.2083 |
| LEAP-Seq n confirming: | 16992 |
| LEAP-Seq n nonconfirming: | 310 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATGCAAGCTCCATCTCAA |
| Suggested primer 2: | CTTGAGAGTTCCCTTGCTGG |