| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059127 |
| Chromosome: | chromosome 6 |
| Location: | 5665206 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286300 | UMM5,COQ5B | (1 of 2) PTHR10108//PTHR10108:SF830 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED; Ubiquinone/menaquinone biosynthesis methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACGATGTCAAAGCTGTCGTCGGGCGCG |
| Internal bar code: | TCACCCACCAGAAGTGGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 752 |
| LEAP-Seq percent confirming: | 99.5045 |
| LEAP-Seq n confirming: | 2008 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCCCCAACATACACATC |
| Suggested primer 2: | GCACACACACTCCATTCACC |