Insertion junction: LMJ.RY0402.059139_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.059139
Chromosome:chromosome 12
Location:8082441
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g551650 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTCAGACGCAACAGGCGCCTGAACGAGA
Internal bar code:TTTATCTCAGCGTGATTTCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:180
LEAP-Seq percent confirming:22.7267
LEAP-Seq n confirming:1922
LEAP-Seq n nonconfirming:6535
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTTATGTCGCGAAAAGGAGC
Suggested primer 2:TTCGTGGGAGTAGTATCGGC