| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059188 |
| Chromosome: | chromosome_2 |
| Location: | 5230977 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g106050 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCATACTGCACGTGTTCATAGAGCTACGG |
| Internal bar code: | GGTGGACTGCATTCGTACATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 99.6464 |
| LEAP-Seq n confirming: | 17188 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTGATGTGTGTCTGGTG |
| Suggested primer 2: | AGCAGCCGCAATAGCAGTAT |