| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059196 |
| Chromosome: | chromosome_8 |
| Location: | 1053317 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362100 | FAL11 | Similar to Flagellar Associated Protein FAP154 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACGCAGGTGTCGAGGCAATCATTTTCCAG |
| Internal bar code: | GAAACCTTTCTTCGCCCCACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1053 |
| LEAP-Seq percent confirming: | 99.1727 |
| LEAP-Seq n confirming: | 27330 |
| LEAP-Seq n nonconfirming: | 228 |
| LEAP-Seq n unique pos: | 161 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTGAGGGCGTCTTCTTT |
| Suggested primer 2: | AGCTGACTTCAGTAGGGGCA |