Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.059196 |
Chromosome: | chromosome_8 |
Location: | 1053326 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g362100 | FAL11 | Similar to Flagellar Associated Protein FAP154 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCTGCGTGCGTCACCTGCAGTCGTAGGGA |
Internal bar code: | GGCCTCGGTTGGGTTCACGGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1062 |
LEAP-Seq percent confirming: | 99.6161 |
LEAP-Seq n confirming: | 13754 |
LEAP-Seq n nonconfirming: | 53 |
LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTGTGAGGGCGTCTTCTTT |
Suggested primer 2: | AGCTGACTTCAGTAGGGGCA |