| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059212 |
| Chromosome: | chromosome 9 |
| Location: | 4750934 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397216 | (1 of 3) K01832 - thromboxane-A synthase (TBXAS1, CYP5A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCAGCAGCAGGCGAGGTGGCGGGCATG |
| Internal bar code: | CGCACATCAGTTGCGTGATCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 99.6777 |
| LEAP-Seq n confirming: | 10823 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCGAATACGCTACACAAG |
| Suggested primer 2: | ACACACACACACACACACGG |