Insertion junction: LMJ.RY0402.059212_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.059212
Chromosome:chromosome 9
Location:4750940
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g397216 (1 of 3) K01832 - thromboxane-A synthase (TBXAS1, CYP5A) CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTGGTTGGGCAACCAGCCATGGGTGTGC
Internal bar code:CGGGTCCGTGTTTCTCAGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:803
LEAP-Seq percent confirming:97.6221
LEAP-Seq n confirming:37565
LEAP-Seq n nonconfirming:915
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCGAATACGCTACACAAG
Suggested primer 2:ACACACACACACACACACGG