| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059232 |
| Chromosome: | chromosome 7 |
| Location: | 2264732 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327650 | SMM25 | (1 of 5) PF05572 - Pregnancy-associated plasma protein-A (Peptidase_M43); S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCACCTTCACTCTCACCTCCACACCCC |
| Internal bar code: | TCGGCCGCAGCCACGCACTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 388 |
| LEAP-Seq percent confirming: | 98.6395 |
| LEAP-Seq n confirming: | 145 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCACCACTCCCAACAGG |
| Suggested primer 2: | ACACACACACACACACCACG |