Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.059244 |
Chromosome: | chromosome_8 |
Location: | 300362 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g358563 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCAGTGAGAAGTCGCTAGTCGCTTACCCA |
Internal bar code: | GCGTTGCTCAGACGCGTGCCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 802 |
LEAP-Seq percent confirming: | 99.2188 |
LEAP-Seq n confirming: | 5207 |
LEAP-Seq n nonconfirming: | 41 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGACATTGTGGCAGTGTGG |
Suggested primer 2: | GGTTGACAAGAGCTGCTTCC |