| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059247 |
| Chromosome: | chromosome 17 |
| Location: | 2828231 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719100 | ZYS1b,ZYS1B | Zygote-specific protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGTGTTAGTTGTCATGCGGTGTTGTGT |
| Internal bar code: | GTGTCACATCAAGATACCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 633 |
| LEAP-Seq percent confirming: | 98.7234 |
| LEAP-Seq n confirming: | 232 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGAAATGCTCTAGCCAC |
| Suggested primer 2: | AACAGCGTAGTATGCCCACC |