| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059259 |
| Chromosome: | chromosome 11 |
| Location: | 1595234 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467761 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTGAAAACAATGAAGTTGCCGGTATCC |
| Internal bar code: | TATCCGCGCGTGCGGGGTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 908 |
| LEAP-Seq percent confirming: | 95.6964 |
| LEAP-Seq n confirming: | 1223 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTATCCACCAACCTGCTCG |
| Suggested primer 2: | CTTCCGCGAAAAAGACGTAG |